Circadian expression of clock genes in mouse macrophages, dendritic cells, and B cells

Brain, Behavior, and Immunity In Press, Accepted Manuscript - Note to users doi:10.1016/j.bbi.2011.10.001 | How to Cite or Link Using DOI   Permissions & Reprints Short Communicaton Circadian expression of clock genes in mouse macrophages, dendritic cells, and B cells Adam C. Silvera, , , Alvaro Arjonaa, Michael E. Hughesb, Michael N. Nitabachb, Erol Fikriga, c Purchase a Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA b Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT, USA c Howard Hughes Medical Institute, Chevy Chase, MD Received 4 August 2011; revised 28 September 2011; Accepted 5 October 2011. Available online 13 October 2011. Abstract In mammals, circadian and daily rhythms influence nearly all aspects of physiology, ranging from behavior to gene expression. Functional molecular clocks have been described in the murine spleen and splenic NK cells. The aim of our study was to investigate the existence of molecular clock mechanisms in other immune cells. Therefore, we measured the circadian changes in gene expression of clock genes (Per1, Per2, Bmal1, and Clock) and clock-controlled transcription factors (Rev-erbα and Dbp) in splenic enriched macrophages, dendritic cells, and B cells in both mice entrained to a light-dark cycle and under constant environmental conditions. Our study reveals the existence of functional molecular clock mechanisms in splenic macrophages, dendritic cells, and B cells. Highlights ► Mouse splenic macrophages, dendritic cells, and B cells possess functional circadian molecular clocks. Keywords: Circadian Rhythm; B Cells; Macrophages; Dendritic Cells; Gene Expression Corresponding author. Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, P.O Box 208022, New Haven, Connecticut 06520-8022, USA. Tel.: (203) 785 4140; fax: (203) 785-6815.