Proteome-wide analysis of protein carboxy termini: C terminomics

Nature Methods 7, 508–511 (1 July 2010) | doi:10.1038/nmeth.1467

As proteome-wide C-terminal sequence analysis has been largely intractable, we developed a polymer-based enrichment approach to profile protein C-terminal peptides by mass spectrometry and identified hundreds of C-terminal peptides in the Escherichia coli proteome. We isotopically labeled GluC protease–digested and undigested samples and identified GluC substrates and their cleavage sites by quantification of neo–C-terminal peptides.